Integration of ATAC-seq and RNA-seq Unravels Chromatin Accessibility during Sex Reversal in Orange-Spotted Grouper (Epinephelus coioides)
نویسندگان
چکیده
منابع مشابه
Brain Anatomy and Histology of Orange Spotted Grouper (Epinephelus coioides)
This research was carried out to identify the different layers and cells of orange spotted grouper brain for further toxicological experiments and defects brought by xenobiotics during exposure periods.The anatomy and histology of the brain of orange spotted grouper was illustrated and compared to mammals and other fishes. The preserved structures of teleosts brain species were different as com...
متن کاملExpression profile of amh/Amh during bi-directional sex change in the protogynous orange-spotted grouper Epinephelus coioides
Gonadal differentiation is tightly regulated by the initial sex determining gene and the downstream sex-related genes in vertebrates. However, sex change in fish can alter the sexual fate from one sex to the other. Chemical-induced maleness in the protogynous orange-spotted grouper is transient, and a reversible sex change occurs after the chemical treatment is withdrawn. We used these characte...
متن کاملCharacterization of c-Jun from orange-spotted grouper, Epinephelus coioides involved in SGIV infection.
The nuclear phosphoprotein c-Jun is a member of the AP1 family of transcription activating complex, can be induced by various extracellular stimuli such as virus infection. In this study, the c-Jun gene (Ec-c-Jun) was cloned from orange-spotted grouper, Epinephelus coioides. The full-length Ec-c-Jun cDNA is composed of 2046 bp and encodes a polypeptide of 328 amino acids with 81% identity of ze...
متن کاملATAC-seq: A Method for Assaying Chromatin Accessibility Genome-Wide.
This unit describes Assay for Transposase-Accessible Chromatin with high-throughput sequencing (ATAC-seq), a method for mapping chromatin accessibility genome-wide. This method probes DNA accessibility with hyperactive Tn5 transposase, which inserts sequencing adapters into accessible regions of chromatin. Sequencing reads can then be used to infer regions of increased accessibility, as well as...
متن کاملIdentification and characterization of DSCAM isoforms isolated from orange-spotted grouper Epinephelus coioides.
The Down syndrome cell adhesion molecule (DSCAM), an immunoglobulin (Ig) superfamily member, was first identified from human and subsequently isolated from both vertebrates and invertebrates. Recent studies have shown that the DSCAM molecule serves diverse functions in neurodevelopment, such as axon guidance and neuronal migration. Most studies on DSCAM, however, have focused on mammals and art...
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ژورنال
عنوان ژورنال: International Journal of Molecular Sciences
سال: 2020
ISSN: 1422-0067
DOI: 10.3390/ijms21082800